Regulation of invertase of Actinomyces viscosus.

نویسندگان

  • R A Kiel
  • J M Tanzer
چکیده

The regulation of diethylaminoethyl-partially purified invertase (EC 3.2.1.26; beta-D-fructofuranoside fructohydrolase) from the 37,000 X g-soluble intracellular fluid of Actinomyces viscosus serotype 2 strain M-100 was studied. Glycolytic intermediates, mono-, di-, and triphosphate nucleotides, inorganic phosphate, and various divalent cations were tested for regulatory effects. Fructose-6-phosphate (F6P) and fructose-1,6-diphosphate (FDP) were found to act as noncompetitive inhibitors of invertase. The Ki values for F6P and FDP were found to be 3.4 and 5.1 mM, respectively. The Hill coefficient for sucrose was 1.03 and remained unchanged in the presence of varying amounts of F6P or FDP.

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عنوان ژورنال:
  • Infection and immunity

دوره 17 3  شماره 

صفحات  -

تاریخ انتشار 1977